GSM2186748: LNCaP DHT + Bicalutamide treated; Homo sapiens; ncRNA-Seq - SRA

SRX1818768: GSM2186748: LNCaP DHT + Bicalutamide treated; Homo sapiens; ncRNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 181.8M spots, 9.3G bases, 5.8Gb downloads

Submitted by: NCBI (GEO)

Study: Investigation of androgen-regulated lncRNAs in prostate cancer cells

PRJNA324393 • SRP076096 • All experiments • All runs

show Abstracthide Abstract

Prostate cancer is the most common cancer in men and androgen receptor (AR) downstream signalings promote prostate cancer cell proliferation. To investigate the AR signaling, we performed directional RNA sequence analysis in AR positive prostate cancer cell line, LNCaP and VCaP. Using Noncode and GENCODE data sets. We identified androgen-regulated long non-coding RNAs (lncRNAs) in prostate cancer cells. Overall design: Directional RNA sequence analysis of androgen-regulated lncRNAs in prostate cancer cells

Sample: LNCaP DHT + Bicalutamide treated

SAMN05201377 • SRS1482651 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ncRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: Total RNA was extracted using ISOGEN reagent. RNA sequencing (RNA-seq) library construction and sequencing was performed according to the manufacturer’s protocol (OP-Hiseq2000-sequencing-cBot-v3.0.) for hiseq2000.

Experiment attributes:

GEO Accession: GSM2186748

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 181.8M spots, 9.3G bases, 5.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR3623700	181,790,127	9.3G	5.8Gb	2016-08-01

ID:: 2600040

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